目的 探讨不同生物黏附材料对三七总皂苷(PNS)微丸体外黏附性和在人工胃液中留存的影响。方法 选择不同生物黏附材料,并进行相应组合制备PNS生物黏附微丸,通过组织留存量法测定PNS生物黏附微丸的体外黏附性,并采用HPLC测定黏附微丸在人工胃液中释放一定时间后留存于微丸中的PNS(三七皂苷R1,人参皂苷Rg1,人参皂苷Rb1)含量。结果 以壳聚糖和壳聚糖-卡波姆组合为生物黏附材料制备的微丸在人工胃液中2 h留存量最大,分别是(27.60±7.45)%和(19.80±3.55)%(R1); (27.01±5.49)%和(19.67±1.39)%(Rg1);(47.07±6.26)%和(51.08±7.44)%(Rb1)。HPMC和HPMC-卡波姆组合黏附性最好,其次是壳聚糖和壳聚糖-卡波姆组合。结论 以壳聚糖和壳聚糖-卡波姆组合为生物黏附材料制备的生物黏附微丸对PNS在人工胃液中降解具有一定的保护作用,且黏附性较好。
Abstract
OBJECTIVE To explore the effects of different bio-adhesive materials on in vitro adhesion and retention in artificial gastric acid fluid of Panax notoginseng saponins(PNS) bio-adhesive pellets.METHODS PNS bio-adhesive pellets were prepared with different bio-adhesive materials.The in vitro adhesion of PNS bio-adhesive pellets was determined by the tissue retention method, using HPLC method to determine the contents of PNS(including notoginseng saponins R1, ginseng saponin Rg1 and ginseng saponin Rb1) retained in the pellets after a certain period of time in artificial gastric acid fluid.RESULTS The contents of PNS retained in PNS bio-adhesive pellets prepared with chitosan and chitosan-carbomer after being placed in artificial gastric acid fluid for 2 h were the largest, which were (27.60±7.45)% and (19.80±3.55)% for R1, (27.01±5.49)% and (19.67±1.39)% for Rg1 and (47.07±6.26)% and (51.08±7.44)% for Rb1, respectively.For in vitro adhesion, HPMC and HPMC-carbomer combination was the best, followed by chitosan and chitosan-carbomer combination.CONCLUSION PNS bio-adhesive pellets prepared with chitosan and chitosan-carbomer combination bio-adhesive materials can protect PNS from degradation in artificial gastric acid fluid and have good adhesion property.
关键词
三七总皂苷 /
生物黏附微丸 /
生物黏附材料 /
体外黏附性 /
药物留存量 /
人工胃液
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Key words
PNS /
bio-adhesive pellet /
bio-adhesive material /
in vitro adhesion /
drug retention /
artificial gastric acid fluid
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中图分类号：
R944
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参考文献
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脚注
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基金
国家自然科学基金资助项目 (81274094)
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